Pour the mixture of lysate and Ni‐NTA agarose in to the column, and permit it to drain either by gravity move or by implementing a vacuum to the bottom of the column. This perform is licensed underneath a Imaginative Commons Attribution 3.0 Unported License. The pictures in this article are https://www.facebook.com/NewTechFind/posts/pfbid0TziLLhy71Gzj4fGXUKbE3PYeFTe7iA3dr8KWckr1uweszvrtmmkT1Wo163JwWVwql